Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland
نویسندگان
چکیده
Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Candida albicans and 17 Candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in Krakow between 2011-2012. The strains were typed by random amplified polymorphic DNA (RAPD) polymerase chain reaction using five primers (CD16AS, HP1247, ERIC-2, OPE-3 and OPE-18). The results of the present investigation revealed a high degree of genetic diversity among the isolates. No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical. The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens. Our study confirmed the high discriminatory power of the RAPD technique in the molecular typing of Candida clinical isolates. This method may be applied to the evaluation of transmission routes of pathogenic fungi on a local level.
منابع مشابه
A Comparative Study on Level of erg11 Gene Expression in Non-albicans Candida Strains Isolated from Patients and Raw Milk
Nowadays, non-albicans Candida are common in human pathogens, and some of these cases were found in milk in the present study. Thanks to its various minerals and vitamins, milk is a good food source for humans and an ideal environment for the growth of microorganisms, including bacteria and fungi. Also, it is an important product in the agricultural sector. Materials and Methods: In the presen...
متن کاملDNA Determination of Candida Species in Blood Cultures of Patients in Oncology Department and Intensive Care Unit in Zanjan Valiasr Hospital, 2017-2018
Background and purpose: Candidiasis is one of the most serious problems in adult and neonatal intensive care units. Molecular techniques play a key role in identifying invasive fungal infections and could be of great benefit beside other mycological diagnostic methods such as smear preparation and blood culture. The aim of this study was to detect candida species isolated from patients in onco...
متن کاملIdentification and antifungal susceptibility pattern of Candida species isolated from patients with nosocomial candiduria
Introduction: Nosocomial candiduria could be due to cystitis, pyelonephritis, or fungusball in the urinary tract system. Several reports have shown candidemia and upper urinarytract involvement as the complications of candiduria. The aim of this study was to assessnosocomial candiduria; identify Candida isolates and determine their drug susceptibilitypattern. Materials and Methods: Urine sample...
متن کاملMolecular epidemiology of candidemia: evidence of clusters of smoldering nosocomial infections.
BACKGROUND Invasive fungal infections pose a serious threat to hospitalized patients worldwide. In particular, the prevalence of clusters of nosocomial infection among patients with candidemia remains unknown. The aim of this study was to investigate the molecular epidemiology of candidemia in a nationwide setting in Iceland during a 16-year period. METHODS The genotypes of all available fung...
متن کاملNational epidemiology of mycoses survey (NEMIS): variations in rates of bloodstream infections due to Candida species in seven surgical intensive care units and six neonatal intensive care units.
Candida species are the fourth most frequent cause of nosocomial bloodstream infections, and 25%-50% occur in critical care units. During an 18-month prospective study period, all patients admitted for > or = 72 hours to the surgical (SICUs) or neonatal intensive care units (NICUs) at each of the participant institutions were followed daily. Among 4,276 patients admitted to the seven SICUs in s...
متن کامل